Powerful Proof

Factors such as stress, poor diet, and environmental toxins can weaken cells’ natural defenses, lower cell energy, damage DNA, and decrease cell performance. All of these can contribute to cellular aging.

Clinical Studies show Vivix® ingredients positively impact four key mechanisms of aging at the cellular level.

1) Cell Defense

Blunts Biological Stress
A clinical study published in The Journal of Endocrinology and Metabolism showed that key ingredients in Vivix blocked a key marker of biological stress response after the consumption of a high-fat fast food breakfast totaling 930 calories.

2) Cell Energy

Increased Energy Production
Vivix increases energy production at the cellular level, which declines as we age. A laboratory study at a leading university showed that key ingredients in Vivix significantly increased mitochondria-the cellular power plants that produce energy.

**In a laboratory study at a leading university, Vivix = resveratrol + proprietary polyphenol blend.

3) Cell Repair

DNA Protection & Repair
Laboratory studies show that key Vivix ingredients help protect against DNA damage, supporting DNA replication for healthy cell function.

Shown in laboratory studies, Vivix protects and repairs DNA, which is assaulted millions of times every day. These assaults can damage the cell’s DNA, creating a “typo” that may compromise cell function and longevity.

4) Cell Performance

Inhibiting The Formation of AGE Proteins
Results from a laboratory study at a leading university showed that key ingredients in Vivix inhibited the formation of AGE proteins. Vivix activates a genetic regulator that helps improve cellular performance and slows the formation of damaging AGE Proteins, which can accumulate and result in cell damage.*

1) Cell Defense References

Ghanim h, Sia Cl, Korzeniewski K, lohano T, Abuaysheh S, Marumganti A, Chaudhuri A, Dandona P. A resveratrol and Polyphenol Preparation Suppresses oxidative and inflammatory Stress Response to a High-Fat, High-Carbohydrate Meal. J Clin Endocrinol Metab.96(5):1409-1414, 2011.

Noratto GD, Angel-Morales G, Talcott ST, Mertens-Talcott Su. Polyphenolics from Açaí (Euterpe Oleracea Mart.) and red Muscadine Grape (Vitis Rotundifolia) Protect human umbilical Vascular Endothelial Cells (huVEC) from Glucose – and lipopolysaccharide (lPS-)induced inflammation and Target MicrornA-126.
J Agric Food Chem 59(14):7999-8012, 2011.

McDougald lr, hofacre C, Mathis G, fuller l, hargrove Jl, Greenspan P, hartle DK. Enhancement of resistance to Coccidiosis and necrotic Enteritis in Broiler Chickens by Dietary Muscadine Pomace. Avian Dis 52(4):646-651, 2008.

Ho l, Chen lh, wang J, Zhao w, Talcott ST, ono K, Teplow D, humala n, Cheng A, Percival SS, ferruzzi M, Janle E, Dickstein Dl, Pasinetti GM. heteroge- neity in red wine Polyphenolic Contents Differentially influences Alzheimer’s Disease-Type neuropathology and Cognitive Deterioration. J Alzheimers Dis 16(1):59-72, 2009.

Bralley EE, hargrove Jl, Greenspan P, hartle DK. Topical Anti-inflammatory Activities of Vitis Rotundifolia (Muscadine Grape) Extracts in the Tetradecanoylphor- bol Acetate Model of Ear inflammation. J Med Food 10(4):636-642, 2007.

Greenspan P, Bauer JD, Pollock Sh, Gangemi JD, Mayer EP, Ghaffar A, hargrove Jl, hartle DK. Anti-inflammatory Properties of the Muscadine Grape (Vitis Rotundifolia).
J Agric Food Chem 53(22):8481-8484, 2005.

Tate P, God J, Bibb r, lu Q, larcom ll. inhibition of Metalloproteinase Activity by fruit Extracts. Cancer Lett 212(2):153-158, 2004.

Ungvari Z, orosz Z, rivera A, labinskyy n, Xiangmin Z, olson S, Podlutsky A, Csiszar A. resveratrol increases Vascular oxidative Stress resistance.
Am J Physiol Heart Circ Physiol 292(5):h2417-h2424, 2007.

Csiszar A, Smith K, labinskyy n, orosz Z, rivera A, ungvari Z. resveratrol Attenuates Tnf-Alpha-induced Activation of Coronary Arterial Endothelial Cells: role of NF-KappaB inhibition. Am J Physiol Heart Circ Physiol 291(4):h1694-h1699, 2006.

Donnelly lE, newton r, Kennedy GE, fenwick PS, leung rh, ito K, russell rE, Barnes PJ. Anti-inflammatory Effects of resveratrol in lung Epithelial Cells: Molecular Mechanisms. Am J Physiol Lung Cell Mol Physiol 287(4):l774-l783, 2004.

Shigematsu S, ishida S, hara M, Takahashi n, Yoshimatsu h, Sakata T, Korthuis rJ. resveratrol, a red wine Constituent Polyphenol, Prevents Superoxide-Dependent inflammatory responses induced by ischemia/reperfusion, Platelet-Activating factor, or oxidants. Free Radic Biol Med 34(7):810-817, 2003.

2) Cell Energy References

lagouge M, Argmann C, Gerhart-hines Z, Meziane h, et al. resveratrol improves Mitochondrial function and Protects Against Metabolic Disease by Activating SirT1 and PGC-1. Cell 127(6):1109-1122, 2006.

Dasgupta B, Milbrandt J. resveratrol Stimulates AMP Kinase Activity in neurons, Proc Natl Acad Sci USA 104(17):7217-7222, 2007.

St-Pierre J, Drori S, uldry M, Silvaggi J, rhee J, Jäger S, handschin C, et al. Suppression of reactive oxygen Species and neurodegeneration by the PGC-1 Transcriptional Coactivators. Cell 127(2):397-408, 2006.

Civitarese A, Carling S, heilbronn l, hulver M, ukropcova B, Deutsch w, Smith S, ravussin E, Calorie restriction increases Muscle Mitochondrial Biogenesis in Healthy Humans. PLoS Medicine 4(3):e76, 2007.

lopez-lluch G, irusta P, navas P, de Cabo r. Mitochondrial Biogenesis and healthy Aging. Exp Gerontol 43(9):813-819, 2008.

3. Cell Repair References

Usha S, irudayam MJ, Malathi r. interaction of resveratrol and Genistein with nucleic Acids. J Biochem Molec Biol 38(2):198-205, 2005.

niture S, Velu C, Smith Q, Bhat J, Srivenugopal K. increased Expression of the MGMT repair Protein Mediated by Cysteine Prodrugs and Chemopreventive Natural Products in Human Lymphocytes and Tumor Lines. Carcinogenesis 28(2):378-389, 2007.

Yang S, irani K, heffron S, Jurnack f, Meyskens f. Alterations in the Expressions of the Apurinic/Apyrimidinic Endonuclease-1/redox factor-1 (APE/ref-1) in human Melanoma and identification of the Therapeutic Potential of resveratrol as an APE/ref-1 inhibitor. Mol Cancer Ther 4(12):1923-1935, 2005.

Zahid M, Gaikwad nw, rogan EG, Cavalieri El. inhibition of Depurinating Estrogen-DnA Adduct formation by natural Compounds. Chem Res Toxicol 20(12): 1947-1953, 2007.

Chakraborty S, roy M, Bhattacharya rK. Prevention and repair of DnA Damage by Selected Phytochemicals as Measured by Single-Cell Gel Electrophoresis. J Environ Pathol Toxicol Oncol 23(3): 215-226, 2004.

Gatz SA, Keimling M, Baumann C, Dörk T, Debatin KM, fulda S, wiesmüller l. resveratrol Modulates DnA Doublestrand Break repair Pathways in an ATM/ATr-p53-and–nbs1-Dependent Manner. Carcinogenesis 29(3): 519-527, 2008.

God JM, Tate P, larcom ll. Anti-Cancer Effects of four Varieties of Muscadine Grape. J Med Food 10(1):54-59, 2007.

4. Cell Performance References

Farrar J, hartle D, hargrove J, Greenspan P. inhibition of Protein Glycation by Skins and Seeds of the Muscadine Grape. BioFactors 30(3):193-200, 2007. hudson T, hartle D, hursting S, nunez n, wang T, Young h, Arany P, Green J. inhibition of Prostate Cancer Growth by Muscadine Grape Skin Extract and Resveratrol through Distinct Mechanisms. Cancer Res 67(17):8396-8405, 2007.

Bralley E, Greenspan P, hargrove J, hartle D. inhibition of hyarluronidase Activity by Vitis Rotundifolia (Muscadine) Berry Seeds and Skins. Pharmaceutical Biology 45(9):667-673, 2007.

Ramasamy r, Vannucci S, Shi Du Yan S, herold K, Yan S, Schmidt A. Advanced Glycation End Products and rAGE: A Common Thread in Aging, Diabetes, Neurodegeneration, and inflammation. Glycobiology 15(7):16r– 28r, 2005.

Mellen PB, Daniel Kr, Brosnihan KB, hansen KJ, herrington DM. Effect of Muscadine Grape Seed Supplementation on Vascular function in Subjects with or At risk for Cardiovascular Disease: A randomized Crossover Trial. J Am Coll Nutr 29(5):469-475, 2010.

Additional Research in Support of Vivix

Scientists around the world have been investigating polyphenolic compounds such as resveratrol and have shown in laboratory studies that these compounds can potentially improve health and well-being.*1

Public health agencies such as the National Institutes of Health (NIH), the National Cancer Institute (NCI), the National Institute on Aging(NIA) as well as the United States Department of Agriculture (USDA) have reviewed and studied the potential lifestyle benefits of phenolic compounds.2

The National Institutes of Health (NIH) spent over $2.4 billion in 2007 alone to further the scientific understanding of the processes of aging. Towards this end, the National Institute on Aging (NIA) has taken the lead to further the understanding of aging and supports laboratory research on resveratrol and improved health.*3,2

A landmark laboratory study from Harvard Medical School and published in Nature in 2006 suggests that resveratrol improves energy balance and may protect against cellular aging processes in mice.*4

Later this study data was converted into a human dose equivalent and published by a research team in a 2007 online and 2008 print journal issue of the Federation of American Societies for Experimental Biology (FASEB). 5,6

More recent studies published this year in PLoS ONE, June 2008 and Cell Metabolism, August 2008 indirectly confirm the potential positive effects and intake levels as well.7,8

Specifically, researchers from the National Institute on Aging and other institutions published in the 2008 issue of Cell Metabolism data confirming that resveratrol may mimic, in laboratory studies, almost all of the effects of dietary or calorie restriction. This process may activate genetic regulators of cellular longevity pathways, which may have positive effects on cellular health and metabolism.*8

Most importantly, the Linus Pauling Institute at Oregon State University and other organizations explain that ‘resveratrol is not known to be toxic or cause adverse effects in humans. Most prominently, safety data from animal studies were published in Toxicoll Sciences in 2004 and in the Journal of Nutrition in 2002.

Furthermore, a recent trial that evaluated the safety of oral resveratrol in ten subjects found a single dose from 0.5 to 5 grams resulted in no serious adverse effects. The study was published in 2007 in Cancer Epidemiol Biomarkers Prev 9,10,11,12

[* These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.]